Title:
Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea.
Creator:
Haqqi, Tariq M.
Anthony, Donald D.
Gupta, Sanjay
Ahmad, Nihal
Lee, M.-S.
Kumar, Ganesh K.
Mukhtar, Hasan
more
Date:
1999
Source(s):
Proceedings of the National Academy of Sciences of the United States of America 1999; 96(8): 4524-9
Proc. Natl. Acad. Sci. U.S.A. 1999; 96(8): 4524-9
Subject(s):
Biological Sciences
Arthritis, Experimental/immunology/pathology/prevention & control
*Flavonoids
Phenols/isolation & purification/therapeutic use
Polymers/isolation & purification/therapeutic use
*Tea
more
Publisher:
The National Academy of Sciences
Format:
text/html
application/pdf
title:
Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea.
creator:
Haqqi, Tariq M.
Anthony, Donald D.
Gupta, Sanjay
Ahmad, Nihal
Lee, M.-S.
Kumar, Ganesh K.
Mukhtar, Hasan
subject:
Biological Sciences
Arthritis, Experimental/immunology/pathology/prevention & control
*Flavonoids
Phenols/isolation & purification/therapeutic use
Polymers/isolation & purification/therapeutic use
*Tea
description:
Identification of common dietary substances capable of affording protection or modulating the onset and severity of arthritis may have important human health implications. An antioxidant-rich polyphenolic fraction isolated from green tea (green tea polyphenols, GTPs) has been shown to possess anti-inflammatory and anticarcinogenic properties in experimental animals. In this study we determined the effect of oral consumption of GTP on collagen-induced arthritis in mice. In three independent experiments mice given GTP in water exhibited significantly reduced incidence of arthritis (33% to 50%) as compared with mice not given GTP in water (84% to 100%). The arthritis index also was significantly lower in GTP-fed animals. Western blot analysis showed a marked reduction in the expression of inflammatory mediators such as cyclooxygenase 2, IFN-gamma, and tumor necrosis factor alpha in arthritic joints of GTP-fed mice. Histologic and immunohistochemical analysis of the arthritic joints in GTP-fed mice demonstrated only marginal joint infiltration by IFN-gamma and tumor necrosis factor alpha-producing cells as opposed to massive cellular infiltration and fully developed pannus in arthritic joints of non-GTP-fed mice. The neutral endopeptidase activity was approximately 7-fold higher in arthritic joints of non-GTP-fed mice in comparison to nonarthritic joints of unimmunized mice whereas it was only 2-fold higher in the arthritic joints of GTP-fed mice. Additionally, total IgG and type II collagen-specific IgG levels were lower in serum and arthritic joints of GTP-fed mice. Taken together our studies suggest that a polyphenolic fraction from green tea that is rich in antioxidants may be useful in the prevention of onset and severity of arthritis.
publisher:
The National Academy of Sciences
date:
1999
type:
Text
format:
text/html
application/pdf
identifier:
PMID: 10200295
PMCID: 16365
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=16365
http://www.ncbi.nlm.nih.gov/sites/entrez?EntrezSystem2.PEntrez.Pubmed.SearchBar.Db=pubmed&EntrezSystem2.PEntrez.Pubmed.SearchBar.Term=10200295[uid]
ksl:pubmedcentral-16365
source:
Proceedings of the National Academy of Sciences of the United States of America 1999; 96(8): 4524-9
Proc. Natl. Acad. Sci. U.S.A. 1999; 96(8): 4524-9
language:
en
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